SPARC antibody (AA 18-302)
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- Target See all SPARC Antibodies
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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Binding Specificity
- AA 18-302
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SPARC antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Osteonectin (ON)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against ON. It has been selected for its ability to recognize ON in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Osteonectin (ON) corresdonding to Ala18~Ile302 (Accession # P07214)
- Isotype
- IgG
- Top Product
- Discover our top product SPARC Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
- Alternative Name
- Osteonectin (SPARC Products)
- Synonyms
- ON antibody, AA517111 antibody, BM-40 antibody, hm:zeh0062 antibody, SPARC antibody, sparc antibody, secreted protein acidic and cysteine rich antibody, secreted acidic cysteine rich glycoprotein antibody, secreted protein, acidic, cysteine-rich (osteonectin) antibody, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog antibody, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog antibody, SPARC antibody, Sparc antibody, sparc antibody, sparc.L antibody, sparc.S antibody
- Background
- SPARC, BM-40, Secreted Protein,Acidic,Cysteine-Rich, Basement-membrane protein 40, Secreted protein acidic and rich in cysteine
- Pathways
- Autophagy
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