PHPT1 antibody (AA 1-124)

Catalog No. ABIN1078437

Product Details anti-PHPT1 Antibody

Target: PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))

Binding Specificity: AA 1-124

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHPT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PHPT1. It has been selected for its ability to recognize PHPT1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Phosphohistidine Phosphatase 1 (PHPT1) corresdonding to Met1~Tyr124 (Accession # Q9DAK9)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PHPT1

Target: PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))

Alternative Name: Phosphohistidine Phosphatase 1 (PHPT1 Products)

Synonyms: wu:fb50e08 antibody, zgc:101086 antibody, MGC131298 antibody, PHP14 antibody, CGI-202 antibody, HSPC141 antibody, RP11-216L13.10 antibody, bA216L13.10 antibody, 1700008C22Rik antibody, Php14 antibody, phosphohistidine phosphatase 1 antibody, phosphohistidine phosphatase 1 L homeolog antibody, 14 kDa phosphohistidine phosphatase-like antibody, LOC424401 antibody, phpt1 antibody, PHPT1 antibody, phpt1.L antibody, LOC100511240 antibody, Phpt1 antibody

Background: PHP14, Phosphohistidine Phosphatase 14 kDa, Sex-Regulated Protein Janus-a, Protein janus-A homolog

Pathways: Positive Regulation of Peptide Hormone Secretion