Trypsin Inhibitor antibody

Catalog No. ABIN107866

Product Details anti-Trypsin Inhibitor Antibody

Target: Trypsin Inhibitor

Reactivity: Lima bean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Trypsin Inhibitor antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Trypsin Inhibitor from other tissues and species may occur but have not been specifically determined.

Purity: Anti-TRYPSIN INHIBITOR is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].

Endotoxin Level: Low Endotoxin : No

Immunogen: Trypsin Inhibitor [Lima Bean]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: TRYPSIN INHIBITOR antibody has been assayed against 1.0 µg of Trypsin Inhibitor [Lima Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:15.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Trypsin Inhibitor

Target: Trypsin Inhibitor

Abstract: Trypsin Inhibitor Products

Synonyms: CRISP11 antibody, LCRISP2 antibody, GRMZM2G447785 antibody, cysteine rich secretory protein LCCL domain containing 2 antibody, trypsin inhibitor antibody, trophoblast Kunitz domain protein 1 antibody, Kazal-type serine proteinase inhibitor 1 antibody, Bowman-Birk type bran trypsin inhibitor antibody, CRISPLD2 antibody, LOC548021 antibody, TKDP1 antibody, Spi1 antibody, LOC100284348 antibody

Background: Synonyms: 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Gene ID: 547831

UniProt: P01070