HLA-DQA2 antibody (AA 40-50)

Catalog No. ABIN1102096

Product Details anti-HLA-DQA2 Antibody

Target: HLA-DQA2 (Major Histocompatibility Complex, Class II, DQ alpha 2 (HLA-DQA2))

Binding Specificity: AA 40-50

Reactivity: Human, Sheep, Cow, Goat

Host: Goat

Clonality: Polyclonal

Conjugate: This HLA-DQA2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HLA-DQA2.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Sheep, Goat, Bovine (100%) Chimpanzee, Zebu (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QSHGPSGQYTH from an internal region of human HLA-DQA2 (NP_064440.1). Percent identity by BLAST analysis: Human, Gorilla, Sheep, Goat, Bovine (100%), Chimpanzee, Zebu (91%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave bands at approx 35kD and 24kD in Human Lymph Node and Tonsil lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 28.0kD according to NP_064440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HLA-DQA2

Target: HLA-DQA2 (Major Histocompatibility Complex, Class II, DQ alpha 2 (HLA-DQA2))

Alternative Name: HLA-DQA2 (HLA-DQA2 Products)

Synonyms: DX-ALPHA antibody, HLA-DXA antibody, major histocompatibility complex, class II, DQ alpha 2 antibody, HLA-DQA2 antibody

Background: Name/Gene ID: HLA-DQA2

Synonyms: HLA-DQA2, DX-ALPHA, DX alpha chain, MHC class II DQA2, HLA-DXA

Gene ID: 3118

NCBI Accession: NP_064440

UniProt: P01906

Pathways: TCR Signaling