IL-27 antibody

Catalog No. ABIN1102483

Product Details anti-IL-27 Antibody

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody is specific for mouse and rat p28 protein. Cross-reactivity with IL-27 from other sources has not been determined.

Purification: Ion exchange chromatography

Sterility: Sterile filtered

Immunogen: This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E. coli) followed by hybridoma development.

Type of Immunogen: Recombinant protein

Clone: 3H12-F10

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:1000)

Usage: IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for IL-27

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Alternative Name: IL27 (IL27 Products)

Synonyms: IL-27 antibody, IL-27A antibody, IL27A antibody, IL27p28 antibody, IL30 antibody, p28 antibody, RGD1561420 antibody, IL27 antibody, IL-27p28 antibody, Il30 antibody, interleukin 27 antibody, IL27 antibody, Il27 antibody

Background: Name/Gene ID: IL27
Family: Interleukin

Synonyms: IL27, IL-27, IL-27 p28 subunit, IL30, Interleukin 27, Interleukin-27 subunit alpha, IL27A, Interleukin 30, IL-27-A, IL27p28, IL-27 subunit alpha, IL-27A, IL27-A, p28

Gene ID: 246778

UniProt: Q8NEV9