PDE4B antibody (AA 724-736)

Catalog No. ABIN1103526

Product Details anti-PDE4B Antibody

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Binding Specificity: AA 724-736

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PDE4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human PDE4B. This antibody is expected to recognize all reported isoforms (NP_002591.2, NP_001032416.1, NP_001032417.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%) Gibbon, Galago, Marmoset, Mouse, Hamster (92%) Rat, Rabbit, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-IDIATEDKSPVDT from the C-terminus of human PDE4B (NP_002591.2, NP_001032416.1, NP_001032417.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%), Gibbon, Galago, Marmoset, Mouse, Hamster (92%), Rat, Rabbit, Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 98kD band observed in Human Uterus lysates (calculated MW of 83.3kD according to NP_002591.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Mhats et al, Endocrinology. 1999 Jul,140(7):3228-37. PMID: 10385419). Recommended concentration: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PDE4B

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Alternative Name: PDE4B (PDE4B Products)

Synonyms: dpde4 antibody, pde4b5 antibody, pdeivb antibody, Dpde4 antibody, R74983 antibody, dunce antibody, PDE4/IVb antibody, Pde4B1 antibody, Pde4B2 antibody, Pde4B3 antibody, Pde4B4 antibody, DPDE4 antibody, PDE4B5 antibody, PDEIVB antibody, phosphodiesterase 4B antibody, phosphodiesterase 4B, cAMP specific antibody, phosphodiesterase 4B S homeolog antibody, pde4b antibody, PDE4B antibody, Pde4b antibody, pde4b.S antibody

Background: Name/Gene ID: PDE4B
Subfamily: PDE - cAMP-specific
Family: Phosphodiesterase

Synonyms: PDE4B, Pde-ivb, PDE32, PDE4B5, PDEIVB, Phosphodiesterase 4b, DPDE4, PDE4B3

Gene ID: 5142

NCBI Accession: NP_002591, NP_001032416, NP_001032417

UniProt: Q07343

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction