ZEB2 antibody (AA 521-534)

Catalog No. ABIN1105104

Product Details anti-ZEB2 Antibody

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Binding Specificity: AA 521-534

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Horse, Dog, Monkey, Xenopus laevis, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This ZEB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ZEB2. This antibody is expected to recognize both reported isoforms (NP_055610.1, NP_001165124.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Xenopus (100%) Zebrafish (93%) Turkey, Stickleback, Pufferfish (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TDSRRQISNIKKEK from an internal region of human ZEB2 (NP_055610.1, NP_001165124.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Xenopus (100%), Zebrafish (93%), Turkey, Stickleback, Pufferfish (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.2 - 0.5 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 100+38kD bands observed in Human Liver lysates (calculated MW of 136kD according to NP_055610.1). Recommended concentration: 0.2-0.5 μg/mL. We believe the two observed bands represent two breakdown fragments.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ZEB2

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Alternative Name: ZEB2 (ZEB2 Products)

Synonyms: ZFHX1B antibody, Zfhx1b antibody, HSPC082 antibody, SIP-1 antibody, SIP1 antibody, SMADIP1 antibody, 9130203F04Rik antibody, D130016B08Rik antibody, Zfx1b antibody, Zfxh1b antibody, mKIAA0569 antibody, XSip1 antibody, sip1 antibody, zfhx1b antibody, si:dkey-52o2.2 antibody, sip1a antibody, wu:fc92g08 antibody, sip1b antibody, zinc finger E-box binding homeobox 2 antibody, zinc finger E-box binding homeobox 2 S homeolog antibody, zinc finger homeobox protein antibody, zinc finger E-box binding homeobox 2a antibody, zinc finger E-box binding homeobox 2b antibody, ZEB2 antibody, Zeb2 antibody, zeb2.S antibody, zeb2 antibody, zeb2a antibody, zeb2b antibody

Background: Name/Gene ID: ZEB2

Synonyms: ZEB2, KIAA0569, SIP1, SMADIP1, ZFX1B, SIP-1, SMAD interacting protein 1, Smad-interacting protein 1, ZFHX1B, Zinc finger homeobox 1b, HSPC082

Gene ID: 9839

NCBI Accession: NP_055610, NP_001165124

UniProt: O60315

Pathways: Tube Formation