C1QA antibody

Catalog No. ABIN1105606

Product Details anti-C1QA Antibody

Target: C1QA (Complement Component 1, Q Subcomponent, A Chain (C1QA))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This C1QA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of Mouse C1q. The monoclonal antibody JL-1 is reactive with the collagen-like region (CLR) only, which is the same region to which autoantibodies in mice and humans are binding. Anti-C1q autoantibodies deposit in glomeruli together with C1q but induce overt renal disease only in the context of glomerular immune complex disease. This provides an explanation why anti-C1q antibodies are especially pathogenic in patients with SLE.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Purified

Immunogen: C1q-/- C57BL/6 mice with Purified Mouse C1q.

Clone: JL-1

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for C1QA

Target: C1QA (Complement Component 1, Q Subcomponent, A Chain (C1QA))

Alternative Name: C1q A Subunit (C1QA Products)

Synonyms: zgc:109903 antibody, C1QA antibody, AI255395 antibody, C1q antibody, complement C1q A chain antibody, complement component 1, q subcomponent, A chain antibody, complement component 1, q subcomponent, alpha polypeptide antibody, C1QA antibody, c1qa antibody, C1qa antibody

Background: C1q is a 459 kDa molecule consisting of three individual polypeptide chains. C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway. Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway. C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen. Furthermore, C1q can also recognize molecular patterns associated with pathogens and it can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis. As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE).Synonyms: Complement 1q, Complement C1q, Complement C1q subcomponent subunit A

Gene ID: 712

NCBI Accession: NP_057075

UniProt: P02745

Pathways: Complement System