Collagen Type I antibody

Catalog No. ABIN1106741

The Rabbit Polyclonal anti-Collagen Type I antibody has been validated for IHC (fro), IF, EIA and DB. It is suitable to detect Collagen Type I in samples from Cow.

Quick Overview for Collagen Type I antibody (ABIN1106741)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB)

Product Details anti-Collagen Type I Antibody

Specificity: Specificity was ascertained by direct ELISA using pure Calf Collagen types I and III for coating microplate wells. No binding to calf serum proteins is revealed at simular dilutions of the antibody. Characteristic immunostaining pictures of frozen sections of bovine kidney and skin are produced to certify absence of crossreactivity with basement membrane collagens (types IV and V).

Cross-Reactivity (Details): Species reactivity (tested):Bovine.
Also reacts to: type III

Purification: Affinity Chromatography: The affinity purified antibody is obtained by binding to immobilized native calf collagen, mixture of type I and III (the antigens used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.

Immunogen: Native Calf Collagen, mixture of type I and III: Antibodies to Calf Collagen type I and III are raised in rabbits which are numerously immunized with extensively purified native collagen type I and III extracted from bovine skin into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1 mL distilled water and add preservative if preferred

Concentration: 0.1 mg/mL

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 282187

NCBI Accession: NP_001029211