FAK antibody (AA 706-1052)

Catalog No. ABIN1107179

The Mouse Monoclonal anti-FAK antibody is suitable to detect FAK in samples from Human, Mouse and Rat. It has been validated for WB, IF and IP.

Quick Overview for FAK antibody (AA 706-1052) (ABIN1107179)

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 12G4

Product Details anti-FAK Antibody

Binding Specificity: AA 706-1052

Specificity: This antibody detects human, mouse and rat FAK in Western blotting, Immunoprecipitation and immunocytostaining. This antibody detects ~125 kDa of human FAK on Westernblotting with whole cell lysates from human cell line such as HeLa. Since active FAK is highly phosphorylated, position of FAK band on westernblotting may varry with its phosphorylation level.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Protein-A Sepharose

Immunogen: GST fusion protein containing human FAK (residues 706-1052 a.a.)

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 50 % glycerol, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: FAK1 / PTK2

Background: FAK (focal adhesion kinase) is a protein tyrosine kinase which colocalizes with integrin receptors at cell-substratum contact sites (focal adhesions). It is thought that enhanced tyrosine phosphorylation of FAK is important for integrin mediated signaling as well as for other signalings. FAK associates with a number of proteins such as Src, Shc, PI-3 kinase, Grb2, Cas, Cas-L, paxilin and transmamblane proteins, which enables FAK to couple to a variety of different intracellular transduction pathways in adherent and non-adherent cells. FAK is involved in anchorage-dependent survival signal through PI-3 kinase and also may play a role in cell growth, migration, cytoskeletal organization.Synonyms: FADK1, FAK, Focal adhesion kinase 1, Protein-tyrosine kinase 2, pp125FAK

Gene ID: 5747

NCBI Accession: NP_722560

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling