Histone H2B antibody

Catalog No. ABIN1107547

This Mouse Monoclonal antibody specifically detects Histone H2B in WB, IF and IP. It exhibits reactivity toward Human, Mouse, Rat and Hamster.

Quick Overview for Histone H2B antibody (ABIN1107547)

Target: Histone H2B

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: C14264

Product Details anti-Histone H2B Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat, hamster

Purification: Protein A agarose

Immunogen: Protein fraction extracted from the mitotic chromosome

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for Histone H2B

Target: Histone H2B

Background: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. Acetylation of histone H2B was suppressed to greater extent than that of the others, with histone H3 being relatively least affected. The analysis of acetylation status of each of the four lysine sites at the N-terminal tail of histone H2B revealed decreases consistent with those observed in the total acetylation patterns, with the K12 and K20 residues being markedly more affected than K5 and K15 residues.Synonyms: H2B Histone Family, HIST1H2B

Molecular Weight: 14 kDa