ENO2/NSE antibody

Catalog No. ABIN110966

Product Details anti-ENO2/NSE Antibody

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific for Human Neuron Specific Enolase (NSE), a glycolytic enzyme that is expressed in neuronal tissue and is released as a result of damage to neural tissue. An increase in serum levels of NSE has been found in patients with tumours such as neuroblastoma. Reacts with a 45 kDa band corresponding to NSE on Immunoblots of brain extracts.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Native NSE protein from Human brain.

Clone: 5G10

Isotype: IgG2b

Application Details

Application Notes: ELISA. Immunohistology on Paraffin Sections: 1/100-1/400. Requires antigen retrieval using heat treatment prior to staining of paraffin sections(Sodium Citrate buffer pH 6.0 is recommended for this purpose). Positive Control: Brain cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % sodium azide as preservative.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: Neuron Specific Enolase (ENO2 Products)

Synonyms: ENO2 antibody, DKFZp459B1817 antibody, NSE antibody, AI837106 antibody, D6Ertd375e antibody, Eno-2 antibody, RNEN3 antibody, eno3 antibody, wu:fc09h05 antibody, zgc:92418 antibody, enolase 2 antibody, enolase 2 (gamma, neuronal) antibody, enolase 2, gamma neuronal antibody, enolase 2, gamma, neuronal antibody, ENO2 antibody, Eno2 antibody, eno2 antibody

Background: Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.Synonyms: 2-phospho-D-glycerate hydro-lyase, ENO2, Enolase 2, Gamma-enolase, NSE, Neural enolase

Gene ID: 2026

NCBI Accession: NP_001966

UniProt: P09104