IGF1 antibody

Catalog No. ABIN111026

The Mouse Monoclonal anti-IGF1 antibody has been validated for RIA, EIA and IHC (fro). It is suitable to detect IGF1 in samples from Human.

Quick Overview for IGF1 antibody (ABIN111026)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1 antibody is un-conjugated

Application: Radioimmunoassay (RIA), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BM23

Product Details anti-IGF1 Antibody

Specificity: The basic IGF-1-like forms (pI>9.0) showed substantial cross-reactivity, as did Rat and Mouse sera. Monoclonal antibody BM23 was capable of abolishing the biological activities of IGF-1 in-vitro bioassays for insulin-like, mitogenic and sulphation activities. Cross-reactivity: ILGF-1:100 % ILGF-2:1 % Proinsulin: 1.4 % MSF: 1 % Insulin: < 0.1 %.Aff. Const.:10e8-10e9 l/mole

Purification: Affinity Chromatography on Protein A.

Immunogen: Purified human IGF-1 conjugated to ovalbumin.

Isotype: IgG1

Application Details

Application Notes: RIA. ELISA. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS buffer with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Insulin-Like Growth Factor I / IGF1

Background: Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.Synonyms: IBP1, IGF-I, MGF, Mechano growth factor, Somatomedin-C

Gene ID: 3479

NCBI Accession: NP_000609

UniProt: P05019

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation