Collagen Type I antibody

Catalog No. ABIN111171

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: Both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5 % binding to collagens II and II in ELISA, RIA or Immunoblotting.

Purification: Ammonium sulfate precipitation and chromatography on DEAE-cellulose

Clone: BDI314

Isotype: IgG

Application Details

Application Notes: ELISA: Apparent binding constant from titration curves is approximately 0.1 nM. Antibodysuitable for use in sandwich ELISA for specific determination of collagen I, employed aseither a capture antibody or as a second, detecting antibody. Immunohistochemical staining of 10 m thick cryostat sections fixed with acetone orethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is notrecommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Store at -20 °C. Avoid multiple freeze/thaw cycles.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody

Background: Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452