CD45.2 antibody (PerCP)

Catalog No. ABIN1112458

Product Details anti-CD45.2 Antibody (PerCP)

Target: CD45.2 (CD45 (CD45.2 Allogen))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45.2 antibody is conjugated to PerCP

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.

Immunogen: B10.S mouse thymocytes and splenocytes

Clone: Ks20-8

Isotype: IgG2a

Application Details

Application Notes: It is recommended for use in flow cytometry. Each lot of this CD45.2 antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is less or equal to 0.25 µg per 10^6 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Comment:

PerCP. Abs/Em: 482/ 678 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for CD45.2

Target: CD45.2 (CD45 (CD45.2 Allogen))

Alternative Name: CD45.2 (CD45.2 Products)

Background: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro blocking of B cell responses, and immunohistochemical staining of acetone-fixed frozen sections.