TER-119/Erythroid Cells antibody (PerCP)

Catalog No. ABIN1112487

Product Details

Target: TER-119/Erythroid Cells

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: PerCP

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: The TER-119 mAb is a component of the \\

Immunogen: Day-14 fetal liver cells from a C57BL/6 mouse

Isotype: IgG2b

Application Details

Application Notes: It is recommended for use in flow cytometry. The TER-119 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. For immunofluorescent staining, the suggested use of this reagent is less or equal tless or equal to 0.25 µg per 10^6 cells in 100 µL volume or 100 µL of whole bloodIt is recommended that the reagent be titrated for optimal performance for each application. Recommended UsageIt is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Comment:

PerCP. Abs/Em 482/ 678 nm.

Reagent Preparation:

1. Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add antibody and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PerCP-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details

Target: TER-119/Erythroid Cells

Background: The TER-119 antibody specifically binds to a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the lineage cocktail used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages.