CDK4 antibody

Catalog No. ABIN112138

This Mouse Monoclonal antibody specifically detects CDK4 in WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CDK4 antibody (ABIN112138)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DCS-156

Product Details anti-CDK4 Antibody

Specificity: DCS-35 reacts specifically with CDK4 protein present predominantly in G1 phase of cell cycle. It does not cross-react with cyclins. In immunohisto-chemical application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) DCS-156 shows distinct nuclear staining, especially in tumor areas of enhanced proliferation.

Purification: Affinity Chromatohgraphy on Protein A.

Immunogen: Human recombinant full-length cdk4 polypeptide.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting (Western blotting). Suitable for Immunohistochemistry on Frozen and Paraffin Embedded Sections (improvedstaining after microwave treatment): 1/10Incubation Time: 1-4 h at RT or overnight at 2-8 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL distilled water.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background: Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.Synonyms: Cell division protein kinase 4, Cyclin-dependent kinase 4, PSK-J3

Gene ID: 1019

NCBI Accession: NP_000066

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size