Collagen Type I antibody

Catalog No. ABIN113956

This Rabbit Polyclonal antibody specifically detects Collagen Type I in IHC (fro), IF, IHC (p), RIA and EIA. It exhibits reactivity toward Mouse.

Quick Overview for Collagen Type I antibody (ABIN113956)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Product Details anti-Collagen Type I Antibody

Specificity: This antibody reacts with Mouse Collagen type I (100 %), Mouse Collagen type II and IV (< 0.1 %), Mouse Collage type III (< 1 %), Human, Chicken and Rat Collagen type I (< 0.1 %, RIA at 1/200 dilution).

Cross-Reactivity (Details): Species reactivity (expected):Human, Chicken and Rat.
Species reactivity (tested):Mouse.

Purification: Affinity Chromatography

Immunogen: Purified collagen type I from Mouse skin

Application Details

Application Notes: Immunflourescence (indirect): 1: 80. Immunohistochemistry on frozen and paraffin sections: 1/200-1/600, 60 min at RT or2-8C over night. ELISA (> 1/200, OD > 500). RIA (> 1/200, OD > 500). Positive control: Mouse skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL sterile distilled water. For further dilution use appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: Phosphate Buffered Solution without stabilizer or preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 12842

NCBI Accession: NP_031768

UniProt: P11087