RAF1 antibody

Catalog No. ABIN114553

This Mouse Monoclonal antibody specifically detects RAF1 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for RAF1 antibody (ABIN114553)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAF1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 410

Product Details anti-RAF1 Antibody

Specificity: This antibody reacts to Raf-1 N-terminal (1-240 a.a.)

Purification: Protein G affinity purified

Immunogen: Purified recombinant Raf-1 (mw:75 kDa)

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot: An antibody concentration of 1 μg/mL will allow visualization of 0.2 g/laneof human Raf-1. A main band (75 kDa) was visible.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water is recommended and to adjust the final concentration to 1.00 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Alternative Name: RAF1

Background: The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf

Gene ID: 5894

NCBI Accession: NP_002871

UniProt: P04049

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling