ELISA. Immunoblotting: Recognizes denatured G-CSFR following SDS-PAGE and blotting of 200 gof cell lysate or following immunoprecipitation with polyclonal antibody followed byimmunoblotting. Western Blot: At an antibody concentration of 1-10 μg/mL will allow visualization of 250 ng/lane of human G-CSFR-MBP. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
In double distilled water to a concentration to 1.00 mg/mL
Buffer
0.01 M PBS, pH 7.2
Handling Advice
Dilute only prior to immediate use
Storage
4 °C
Storage Comment
Store the antibody (undiluted) at 2-8 °C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
The protein encoded by the GCSF Receptor gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, of which three are membrane-bound and the one secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.Synonyms: G-CSF Receptor, G-CSF-R, GCSF Receptor, GCSFR, Granulocyte colony-stimulating factor receptor