GZMB antibody (N-Term)

Catalog No. ABIN115414

This Rabbit Polyclonal antibody specifically detects GZMB in IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GZMB antibody (N-Term) (ABIN115414)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GZMB Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts with a 32 kDa protein.
Cellular Localization: Cytoplasmic

Purification: Purified

Immunogen: A synthetic peptide from the N-terminus of human Granzyme B.

Application Details

Application Notes: Immunohistochemistry on Paraffin Embedded Tissues: Use 1/50-1100 dilution in an ABC method (30 minutes at room temperature)Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM Citrate Buffer, pH 6.0 containing Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Background: Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned. Human granzymes B is involved in target cell apoptosis during lymphocyte-mediated cytotoxicity. This antibody is useful in the localization of granzyme B containing lytic granules and for the characterization of activated CTLs or NK cells.Synonyms: CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Granzyme-2, Lymphocyte protease, SECT, T-cell serine protease 1-3E

Gene ID: 3002

NCBI Accession: NP_004122

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis