IGF1 antibody (Biotin)

Catalog No. ABIN116055

This anti-IGF1 antibody is a Rabbit Polyclonal antibody detecting IGF1 in WB and EIA. Suitable for Human.

Quick Overview for IGF1 antibody (Biotin) (ABIN116055)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IGF1 Antibody (Biotin)

Specificity: This antibody reacts with Insulin-like Growth Factor 1

Purification: Affinity Chromatography.

Immunogen: E.coli derived recombinant Human IGF-I (Cat#PA071)

Application Details

Application Notes: Direct ELISA: To detect Human IGF-I by direct ELISA (using 100 μL/well antibody solution) aconcentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I. Sandwich ELISA: To detect Human IGF-I by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. Used inconjunction with Polyclonal anti-Human IGF-I (PP1020P1 or PP1020P2) as a Captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I. Western blot: To detect Human IGF-I by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Hunman IGF-I is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Insulin-Like Growth Factor I / IGF1

Background: Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.Synonyms: IBP1, IGF-I, MGF, Mechano growth factor, Somatomedin-C

Gene ID: 3479

NCBI Accession: NP_001104755

UniProt: P05019

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation