IGF2
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), aconcentration of 3.1 - 4.6 μg/mL of this antibody is required. ElisaIndirect: To detect hIGF-II by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. Sandwich: To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanIGF-II as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIGF-II. Western BlotTo detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Restrictions
For Research Use only
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2
Handling Advice
This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Target
IGF2
(Insulin-Like Growth Factor 2 (IGF2))
Alternative Name
Insulin-Like Growth Factor II / IGF2 (IGF2 Products)
The IGF2 gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is expressed only from the paternally inherited allele and is an imprinted gene which is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types.Synonyms: IGF-II, Somatomedin-A