IL-10 antibody
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- Target See all IL-10 (IL10) Antibodies
- IL-10 (IL10) (Interleukin 10 (IL10))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IL-10 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
- Specificity
- Recognizes Human IL-10. Other species not tested.
- Purification
- Affinity Chromatography employing immobilized hIL-10 matrix
- Immunogen
- Highly pure (>98%) E.coli derived recombinant Human IL-10 (Cat.-No PA084)
- Top Product
- Discover our top product IL10 Primary Antibody
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- Application Notes
- Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman IL-10 (1.0 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. Indirect ELISA: To detect Human IL-10 by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof at least 0.2-0.4 ng/well of recombinant Human IL-10. Sandwich ELISA: To detect Human IL-10 by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human IL-10 (PP1032B1 or
- Restrictions
- For Research Use only
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- Reconstitution
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- IL-10 (IL10) (Interleukin 10 (IL10))
- Alternative Name
- Interleukin-10 / IL10 (IL10 Products)
- Synonyms
- CSIF antibody, GVHDS antibody, IL-10 antibody, IL10A antibody, TGIF antibody, Il-10 antibody, IL10X antibody, interleukin 10 antibody, IL10 antibody, Il10 antibody
- Background
- Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73 % homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.Synonyms: CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF
- Molecular Weight
- approx. 150 KDa
- Gene ID
- 3586
- NCBI Accession
- NP_000563
- UniProt
- P22301
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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