Direct ELISA: To detect Human SCGF-Beta by Direct ELISA (using 100 μL/well) aconcentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated antibody allowsthe detection of at least 0.2-0.4 ng/well of recombinant Human SCGF-Beta. Sandwich ELISA: To detect Human SCGF-Beta by Sandwich ELISA (using 100 μL/well) thisantibody can be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction withpurified anti-Human SCGF beta antibody (PP1106P1 or PP1106P2) as a Capture antibody,allows the detection of at least 0.2-0.4 ng/well of recombinant Human SCGF-Beta. Western Blot: To detect Human SCGF-Beta by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hSCGF-Beta is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Target
CLEC11A
(C-Type Lectin Domain Family 11, Member A (CLEC11A))
This cytokine stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. It acts synergistically with other cytokines, including IL3, GCSF, GMCSF and FLT3 ligand and suppresses SCF-stimulated erythrocyte proliferation.Synonyms: C-type lectin domain family 11 member A, C-type lectin superfamily member 3, CLECSF3, LSLCL, Lymphocyte secreted C-type lectin, Stem cell growth factor, p47