TNFSF13B
Reactivity: Human
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA(Direct): To detect hBAFF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBAFF. (Sandwich): To detect hBAFF by sandwich ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hBAFF. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a
Restrictions
For Research Use only
Reconstitution
Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Target
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
Members of the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF also signals through a third TNF receptor BAFFR/BR3. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain.Synonyms: B cell-activating factor, B lymphocyte stimulator, BAFF, BLYS, Dendritic cell-derived TNF-like molecule, TALL1, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, Tumor necrosis factor ligand superfamily member 13B, ZTNF4