AIMP1
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
3H5
unconjugated
Application Notes
ELISA: To detect hEMAP-II by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthEMAP-II. Western Blot: To detect hEMAP-II by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEMAP-II is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Target
AIMP1
(Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))