IL22
Reactivity: Human
WB
Host: Rabbit
Polyclonal
RB34065
unconjugated
Application Notes
ELISADirect: To detect Human IL-22 by direct ELISA (using 100 μL/well) a concentration of approx. 1.0 μg/mL of this antibody is required. The Biotinylated antibody allows the detection of atleast 0.2-0.4 ng/well of recombinant Human IL-22. Sandwich: To detect Human IL-22 by Sandwich ELISA (using 100 μL/well) a concentration of0.25-1.0 μg/mL of this antibody is required. The Biotinylated antibody, in conjuction withanti-Human IL22 (PP1224P1 or PP1224P2) as a capture antibody, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human IL-22. Western blot: To detect Human IL-22 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human IL-22 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with sterile PBS solution containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
IL22 is a cytokine structurally related to IL10 and is upregulated in activated T cells. IL22 signals through a receptor complex that includes CRF2-4 and a new member of the class II cytokine receptor family (also known as the interferon receptor family), IL22R. CRF2-4 has previously been demonstrated to be a functional component of the IL-10 signaling complex. IL22 is able to suppress IL4 production, this may have therapeutic potential, particularly in the treatment of asthma.Synonyms: Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF