Western blotting: 1/250. Immunoprecipitation. ELISA. Electrophoretic Mobility Shift Assay: using 0.5 to 1.0 l per assay. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against RFX5 (C-terminal specific) from a variety of fibroblast and B-cell lysates ata dilution of 1: 250 to 1: 500 followed by reaction with Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Goat). Anti-RFX5 (C-terminal specific) is suitable for thedetection by immunoblot of human RFX5 a shows a 75 kDa band. This product was alsotested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5to 1.0 l per assay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1 mg/mL (by UV absorbance at 280 nm)
Buffer
0.01 M Na phosphate, 0.11 M NaCl, pH 7.4 with 0.02 % Sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
regulatory factor X5 antibody, regulatory factor X, 5 (influences HLA class II expression) antibody, RFX5 antibody, Rfx5 antibody
Background
Human RFX5 from a variety of fibroblast and B-cell lysates. Recognition is at the C-terminal region. RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.Synonyms: DNA-binding protein RFX5, Regulatory factor X 5