ALPI
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 μg of Alkaline Phosphatase [Calf intestine] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 2,000 to 1: 8,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
85.0 mg/mL (by Refractometry)
Buffer
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 without preservatives.
Preservative
Without preservative
Handling Advice
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage
-20 °C
Storage Comment
Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.Synonyms: Intestinal alkaline phosphatase