NLRP3 antibody (Pyrin Domain)

Catalog No. ABIN1169100

Cited in 60+ publications. The Mouse Monoclonal anti-NLRP3 antibody (Clone Cryo-2) (ABIN1169100) specifically detects NLRP3 in WB, IHC, IF and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for NLRP3 antibody (Pyrin Domain) (ABIN1169100)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: Cryo-2

Product Details anti-NLRP3 Antibody

Key Features:

• NLRP3 antibody for reliable recognition of mouse and human NLRP3/NALP3
• This un-conjugated monoclonal antibody has 60 PubMed citations

Binding Specificity: AA 1-93, Pyrin Domain

Specificity: Recognizes mouse and human NLRP3/NALP3.

Cross-Reactivity: Human, Mouse (Murine)

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse NLRP3/NALP3 (pyrin domain) (aa 1-93).

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-NLRP3 antibody (ABIN1169100)

Zhu, Tang: "LncRNA Gm14205 induces astrocytic NLRP3 inflammasome activation via inhibiting oxytocin receptor in postpartum depression." in: Bioscience reports, Vol. 40, Issue 8, (2020) (PubMed).

Wong, Lewis, Doherty, Shi, Cashikar, Amelianchik, Tymchuk, Sullivan, Qian, Covey, Petsko, Holtzman, Paul, Luo: "25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner." in: Journal of neuroinflammation, Vol. 17, Issue 1, pp. 192, (2020) (PubMed).

Hoyle, Redondo-Castro, Cook, Tzeng, Allan, Brough, Lemarchand: "Hallmarks of NLRP3 inflammasome activation are observed in organotypic hippocampal slice culture." in: Immunology, Vol. 161, Issue 1, pp. 39-52, (2020) (PubMed).

Shrivastava, Visoso-Carvajal, Garcia-Cordero, Leon-Juarez, Chavez-Munguia, Lopez, Nava, Villegas-Sepulveda, Cedillo-Barron: "Dengue Virus Serotype 2 and Its Non-Structural Proteins 2A and 2B Activate NLRP3 Inflammasome." in: Frontiers in immunology, Vol. 11, pp. 352, (2020) (PubMed).

Wang, Hu, Wu, Feng, Li, Liu, Wang, Chen, Tian, Xiao, Zhang, Shereen, Chen, Pan, Wan, Wu, Wu: "STING promotes NLRP3 localization in ER and facilitates NLRP3 deubiquitination to activate the inflammasome upon HSV-1 infection." in: PLoS pathogens, Vol. 16, Issue 3, pp. e1008335, (2020) (PubMed).

Lai, Hsu, Lee, Chiang: "Microtubule-Mediated NLRP3 Inflammasome Activation Is Independent of Microtubule-Associated Innate Immune Factor GEF-H1 in Murine Macrophages." in: International journal of molecular sciences, Vol. 21, Issue 4, (2020) (PubMed).

Wooff, Fernando, Wong, Dietrich, Aggio-Bruce, Chu-Tan, Robertson, Doyle, Man, Natoli: "Caspase-1-dependent inflammasomes mediate photoreceptor cell death in photo-oxidative damage-induced retinal degeneration." in: Scientific reports, Vol. 10, Issue 1, pp. 2263, (2020) (PubMed).

Svensson, Andersson, Manouchehrian, Yang, Deierborg: "Voluntary running does not reduce neuroinflammation or improve non-cognitive behavior in the 5xFAD mouse model of Alzheimer's disease." in: Scientific reports, Vol. 10, Issue 1, pp. 1346, (2020) (PubMed).

Cai, Ge, Mennone, Hoque, Ouyang, Boyer: "Inflammasome Is Activated in the Liver of Cholestatic Patients and Aggravates Hepatic Injury in Bile Duct-Ligated Mouse." in: Cellular and molecular gastroenterology and hepatology, Vol. 9, Issue 4, pp. 679-688, (2020) (PubMed).

Dionísio, Oliveira, Gaspar, Gama, Castro-Caldas, Amaral, Rodrigues: "Ablation of RIP3 protects from dopaminergic neurodegeneration in experimental Parkinson's disease." in: Cell death & disease, Vol. 10, Issue 11, pp. 840, (2020) (PubMed).

Du, Lu, Wang, Ding, Wu, Hu: "The pore-forming subunit Kir6.1 of the K-ATP channel negatively regulates the NLRP3 inflammasome to control insulin resistance by interacting with NLRP3." in: Experimental & molecular medicine, Vol. 51, Issue 8, pp. 1-13, (2020) (PubMed).

Hoss, Mueller, Rojas Ringeling, Rodriguez-Alcazar, Brinkschulte, Seifert, Stahl, Broderick, Putnam, Kolodner, Canzar, Geyer, Hoffman, Latz: "Alternative splicing regulates stochastic NLRP3 activity." in: Nature communications, Vol. 10, Issue 1, pp. 3238, (2020) (PubMed).

Sharif, Wang, Wang, Magupalli, Andreeva, Qiao, Hauenstein, Wu, Núñez, Mao, Wu: "Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome." in: Nature, Vol. 570, Issue 7761, pp. 338-343, (2020) (PubMed).

Xue, Xi, Liu, Guo, Zhang, Zhang, Li, Yang, Zhou, Yang, Zhang, Zhang, Gu, Yang, Da, Yao, Duo, Zhang: "miR-21 promotes NLRP3 inflammasome activation to mediate pyroptosis and endotoxic shock." in: Cell death & disease, Vol. 10, Issue 6, pp. 461, (2020) (PubMed).

Huang, Feng, Jiang, Li, Xiang, Guo, Yang, Fei, Guo, Zheng, Wu, Chen: "VSIG4 mediates transcriptional inhibition of Nlrp3 and Il-1β in macrophages." in: Science advances, Vol. 5, Issue 1, pp. eaau7426, (2020) (PubMed).

Liu, Lu, Chen, Muthuraj, Li, Pattabiraman, Zempleni, Kachman, Natarajan, Yu: "Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice." in: Nutrients, Vol. 12, Issue 2, (2020) (PubMed).

Deng, Guo, Tam, Johnson, Brickey, New, Lenox, Shi, Golenbock, Koller, McKinnon, Beutler, Ting: "Platelet-activating factor (PAF) mediates NLRP3-NEK7 inflammasome induction independently of PAFR." in: The Journal of experimental medicine, Vol. 216, Issue 12, pp. 2838-2853, (2020) (PubMed).

Sanchez-Lopez, Zhong, Stubelius, Sweeney, Booshehri, Antonucci, Liu-Bryan, Lodi, Terkeltaub, Lacal, Murphy, Hoffman, Tiziani, Guma, Karin: "Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production." in: Cell metabolism, Vol. 29, Issue 6, pp. 1350-1362.e7, (2020) (PubMed).

Kiyohara, Sujino, Teratani, Miyamoto, Arai, Nomura, Harada, Aoki, Koda, Mikami, Mizuno, Naganuma, Hisamatsu, Kanai: "Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells." in: Cellular and molecular gastroenterology and hepatology, Vol. 7, Issue 1, pp. 135-156, (2019) (PubMed).

Hafner-Bratkovič, Sušjan, Lainšček, Tapia-Abellán, Cerović, Kadunc, Angosto-Bazarra, Pelegrin, Jerala: "NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway." in: Nature communications, Vol. 9, Issue 1, pp. 5182, (2019) (PubMed).

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3/NALP3

Background: The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

UniProt: Q8R4B8

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome