NLRP3 antibody (Pyrin Domain)

Catalog No. ABIN1169101

This Mouse Monoclonal antibody specifically detects NLRP3 in WB. It exhibits reactivity toward Mouse. It has been mentioned in 4+ publications

Quick Overview for NLRP3 antibody (Pyrin Domain) (ABIN1169101)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: Cryo-1

Product Details anti-NLRP3 Antibody

Binding Specificity: AA 1-93, Pyrin Domain

Specificity: Recognizes mouse NLRP3/NALP3.

Cross-Reactivity: Mouse (Murine)

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse NLRP3/NALP3 (pyrin domain) (aa 1-93).

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-NLRP3 antibody (ABIN1169101)

Dey, Joshi, Oliveira, Pereira, Guimarães-Costa, Serafim, de Castro, Coutinho-Abreu, Bhattacharya, Townsend, Aslan, Perkins, Karmakar, Ismail, Karetnick, Meneses, Duncan, Nakhasi, Valenzuela, Kamhawi: "Gut Microbes Egested during Bites of Infected Sand Flies Augment Severity of Leishmaniasis via Inflammasome-Derived IL-1β." in: Cell host & microbe, Vol. 23, Issue 1, pp. 134-143.e6, (2018) (PubMed).

Li, Ju, Hsieh, Dong, Chen, Hua, Chen: "A synthetic cationic antimicrobial peptide inhibits inflammatory response and the NLRP3 inflammasome by neutralizing LPS and ATP." in: PLoS ONE, Vol. 12, Issue 7, pp. e0182057, (2017) (PubMed).

He, Wan, Hu, Chen, Wang, Huang, Yang, Zhong, Han: "Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion." in: Cell research, Vol. 25, Issue 12, pp. 1285-98, (2016) (PubMed).

Dashdorj, Jyothi, Lim, Jo, Nguyen, Ha, Yoon, Kim, Park, Murphy, Kim: "Mitochondria-targeted antioxidant MitoQ ameliorates experimental mouse colitis by suppressing NLRP3 inflammasome-mediated inflammatory cytokines." in: BMC medicine, Vol. 11, pp. 178, (2013) (PubMed).

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3/NALP3

Background: The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

UniProt: Q8R4B8

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome