Goat anti-Ferret IgG (Chain gamma) Antibody (HRP)

Details for Product No. ABIN117102, Supplier: Log in to see
Antigen
Epitope
Chain gamma
5081
1879
1842
1048
1021
847
326
308
35
34
31
12
8
7
3
3
2
1
1
1
1
1
1
1
Reactivity
Ferret
2878
2598
2171
1546
1387
751
599
453
426
389
366
355
295
278
265
145
131
113
41
30
28
26
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
Host
Goat
6462
4916
1656
986
521
356
93
67
51
18
8
5
5
5
2
1
1
Clonality
Polyclonal
Conjugate
HRP
2210
1817
1736
1282
772
695
446
300
219
171
165
141
137
129
110
107
77
75
71
65
65
56
55
54
54
53
53
53
53
53
53
52
50
40
37
35
23
19
18
17
17
15
15
15
14
14
14
14
12
12
11
10
10
10
10
10
9
9
9
9
9
8
8
8
8
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen Ferret IgG gamma (gamma chain)
Purification Prepared from monospecific antiserum by Immunoaffinity Chromatography using Ferret IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Ferret Immunoglobulin G
Application Notes Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/5,000.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL of deionized water or equivalent.
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Thimerisol as preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -28 °C or below.
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).