Histone antibody (Biotin)
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- Target
- Histone
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Specificity
- It has been selected for its ability to recognize HIS in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
- The antibody is a rabbit polyclonal antibody raised against HIS conjugated to biotin.
- Isotype
- IgG
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- Histone
- Alternative Name
- Histone (HIS)
- Synonyms
- Histone H2A antibody, Histone H2B 1 antibody, Histone H3 antibody, Probable histone H2B 4 antibody, Histone H2B 2 antibody, Histone antibody, his-16 antibody, his-12 antibody, his-11 antibody, his-9 antibody, his-43 antibody, his-61 antibody, his-48 antibody, his-57 antibody, his-22 antibody, his-4 antibody, his-3 antibody, his-42 antibody, his-66 antibody, his-65 antibody, Histone antibody
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