GLB1 antibody (AA 68-219)

Catalog No. ABIN1172543

Product Details anti-GLB1 Antibody

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Binding Specificity: AA 68-219

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Galactosidase Beta (GLb)

Specificity: The antibody is a rabbit polyclonal antibody raised against GLb. It has been selected for its ability to recognize GLb in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Galactosidase Beta (GLb) corresdonding to Arg68~Thr219 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-GLB1 antibody (ABIN1172543)

Löwenau, Zoschke, Brodwolf, Volz, Hausmann, Wattanapitayakul, Boreham, Alexiev, Schäfer-Korting: "Increased permeability of reconstructed human epidermis from UVB-irradiated keratinocytes." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 116, pp. 149-154, (2018) (PubMed).

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: Galactosidase Beta (GLB1 Products)

Synonyms: EBP antibody, ELNR1 antibody, MPS4B antibody, DER antibody, XR antibody, 0610038K04Rik antibody, 1810027P18Rik antibody, glb antibody, CG9092 antibody, Dmel\\CG9092 antibody, beta-GAL antibody, beta-Gal-1 antibody, beta-gal antibody, gal antibody, lacZ-1 antibody, AW125515 antibody, Bge antibody, Bgl antibody, Bgl-e antibody, Bgl-s antibody, Bgl-t antibody, Bgs antibody, Bgt antibody, C130097A14Rik antibody, BGAL antibody, zgc:110823 antibody, ECK0341 antibody, JW0335 antibody, GLB antibody, galactosidase beta 1 antibody, dicarbonyl and L-xylulose reductase antibody, dicarbonyl L-xylulose reductase antibody, beta-D-galactosidase antibody, beta-galactosidase antibody, beta galactosidase antibody, galactosidase, beta 1 antibody, beta-galactosidase-like antibody, GLB1 antibody, DCXR antibody, Dcxr antibody, lacZ antibody, SSP_RS00525 antibody, lacH antibody, ECL_03691 antibody, Gal antibody, Glb1 antibody, glb1 antibody, VV2_1327 antibody, LOC100056371 antibody

Background: GL-B, b-GAL, bGAL, ELNR1, EBP, GLB1, Lactase, Elastin Receptor 1,67 kDa, Elastin Receptor 1(67kD), Acid Beta-Galactosidase

Pathways: Glycosaminoglycan Metabolic Process