CPE antibody (AA 318-476)

Catalog No. ABIN1176423

Product Details anti-CPE Antibody

Target: CPE (Carboxypeptidase E (CPE))

Binding Specificity: AA 318-476

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Carboxypeptidase E (CPE)

Specificity: The antibody is a rabbit polyclonal antibody raised against CPE. It has been selected for its ability to recognize CPE in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Carboxypeptidase E (CPE) corresdonding to Ala318~Phe476 (Accession # P16870)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CPE

Target: CPE (Carboxypeptidase E (CPE))

Alternative Name: Carboxypeptidase E (CPE Products)

Synonyms: CPE antibody, CPH antibody, Cph-1 antibody, Cph1 antibody, R74677 antibody, fat antibody, CARBE antibody, Cph antibody, si:ch211-198g9.2 antibody, zgc:85981 antibody, carboxypeptidase E antibody, carboxypeptidase E L homeolog antibody, CPE antibody, cpe antibody, Cpe antibody, cpe.L antibody

Background: CPH, Enkephalin Convertase, Carboxypeptidase H, Prohormone-processing carboxypeptidase

Pathways: Peptide Hormone Metabolism, Synaptic Membrane