LCK antibody (N-Term) (Alexa Fluor 488)

Catalog No. ABIN1176917

Product Details anti-LCK Antibody (Alexa Fluor 488)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LCK antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human N-terminal Lck

Clone: MOL 171

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-LCK antibody (Alexa Fluor 488) (ABIN1176917)

Veillette, Latour, Davidson: "Negative regulation of immunoreceptor signaling." in: Annual review of immunology, Vol. 20, pp. 669-707, (2002) (PubMed).

Johnson, Bromley, Dustin, Thomas: "A supramolecular basis for CD45 tyrosine phosphatase regulation in sustained T cell activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 18, pp. 10138-43, (2000) (PubMed).

Nakashima, Pu, Hamaguchi, Iwamoto, Rahman, Zhang, Kato, Ohkusu, Katano, Yoshida: "Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3511-20, (1993) (PubMed).

Moroi, Koga, Nakamura, Ohtsu, Kimura, Nomoto: "Accumulation of p60lck in HTLV-I-transformed T cell lines detected by an anti-lck monoclonal antibody, MOL 171." in: Japanese journal of cancer research : Gann, Vol. 82, Issue 8, pp. 909-15, (1991) (PubMed).

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: Lck (LCK Products)

Synonyms: zgc:136695 antibody, LCK antibody, Hck-3 antibody, Lsk antibody, Lskt antibody, p56 antibody, p56Lck antibody, LSK antibody, YT16 antibody, p56lck antibody, pp58lck antibody, P56LCK antibody, tkl antibody, Lck1 antibody, Lcktkr antibody, LCK proto-oncogene, Src family tyrosine kinase antibody, lymphocyte protein tyrosine kinase antibody, lck antibody, LCK antibody, Lck antibody

Background: Lck is a member of the Src family of cytoplasmic protein-tyrosine kinases (PTKs) that is normally expressed exclusively in lymphoid cells, primarily T lymphocytes and NK cells. A low level of expression has been detected in B lymphocytes, but its function in B cells is unknown. Its expression in other leukocytes is not well defined. Members of the Src family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell-surface glycoproteins of T lymphocytes, which recognize antigen presenting cells via their surface MHC class II and class I molecules, respectively. The catalytic activity of Lck is regulated by both kinases and phosphatases that control the phosphorylation states of two tyrosine residues that have opposing effects. Repression of Lck's catalytic activity occurs via phosphorylation at tyrosine 505 (Y505), located near the carboxy terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs, and its dephosphorylation is mediated by the protein tyrosine phosphatase, CD45. When Lck is phosphorylated at this site, it assumes a folded tertiary structure which is enzymatically inactive. When CD45 dephosphorylates it at Y505, Lck is able to autophosphorylate its Y394, which leads to conformational changes in the catalytic domain that induce kinase activity. However, it has been observed that the inhibitory effect of the phosphorylated Y505 can be overcome by direct engagement of Lck's SH3 domain and that both Y394 and Y505 are phosphorylated together in cells activated by hydrogen peroxide. Activated Lck phosphorylates the ITAMs (Immunoreceptor-based Tyrosine Activation Motifs) of the T cell receptor (TCR) and thus is critical for activation and development of T lymphocytes. The interactions of Lck, Csk, CD45, CD4 or CD8, and TCR are only a small part of a complex immunoregulatory cascade that involves additional substrates for Csk and CD45, other enzymes, adhesion molecules, adaptor proteins, and specialized membrane microdomains. The MOL 171 monoclonal antibody recognizes the 56- and 60-kDa forms of human Lck protein, regardless of phosphorylation status. It cross reacts with mouse Lck.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling