IL1R1 antibody

Catalog No. ABIN1176949

This Rat Monoclonal antibody specifically detects IL1R1 in WB, IP, FACS and BR. It exhibits reactivity toward Mouse. It has been mentioned in 2+ publications

Quick Overview for IL1R1 antibody (ABIN1176949)

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL1R1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: 35F5

Product Details anti-IL1R1 Antibody

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Since this antigen may be expressed at low density on the cell surface, investigators may wish to perform staining using Biotin Goat Anti-Rat Ig (Cat. No. 554014) coupled with a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061).
Western Blot: The 35F5 antibody has been reported to be useful for western blotting of non-reduced proteins and being non-reactive to reduced proteins.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL1R1 antibody (ABIN1176949)

McIntyre, Stepan, Kolinsky, Benjamin, Plocinski, Kaffka, Campen, Chizzonite, Kilian: "Inhibition of interleukin 1 (IL-1) binding and bioactivity in vitro and modulation of acute inflammation in vivo by IL-1 receptor antagonist and anti-IL-1 receptor monoclonal antibody." in: The Journal of experimental medicine, Vol. 173, Issue 4, pp. 931-9, (1991) (PubMed).

Chizzonite, Truitt, Kilian, Stern, Nunes, Parker, Kaffka, Chua, Lugg, Gubler: "Two high-affinity interleukin 1 receptors represent separate gene products." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 20, pp. 8029-33, (1989) (PubMed).

Target Details for IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: CD121a

Background: The 35F5 antibody reacts with the type-I interleukin (IL-1) receptor (CD121a) expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80-kDa type-I transmembrane glycoprotein, a member of the Ig superfamily which mediates the inflammatory responses of leukocytes to IL-1. mAb 35F5 is capable of blocking the binding of IL-1alpha, IL-1beta, and IL-1 receptor antagonist to the type-I IL-1 receptor. The 35F5 mAb is highly specific for the type-I IL-1 receptor and does not react with the type-II IL-1 receptor (CD121b) which is present on murine macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.
Synonyms: IL-1 Receptor, Type I/p80

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints