KIT antibody

Catalog No. ABIN1176965

There are 20+ publications for this product available. The Rat Monoclonal anti-KIT antibody is suitable to detect KIT in samples from Mouse. It has been validated for FACS and BR.

Quick Overview for KIT antibody (ABIN1176965)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This KIT antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: ACK45

Product Details anti-KIT Antibody

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse bone marrow mast cells

Isotype: IgG2b kappa

Application Details

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-KIT antibody (ABIN1176965)

Austen, Boyce: "Mast cell lineage development and phenotypic regulation." in: Leukemia research, Vol. 25, Issue 7, pp. 511-8, (2001) (PubMed).

Domen, Weissman: "Hematopoietic stem cells need two signals to prevent apoptosis; BCL-2 can provide one of these, Kitl/c-Kit signaling the other." in: The Journal of experimental medicine, Vol. 192, Issue 12, pp. 1707-18, (2001) (PubMed).

Mirmonsef, Shelburne, Fitzhugh Yeatman, Chong, Ryan: "Inhibition of Kit expression by IL-4 and IL-10 in murine mast cells: role of STAT6 and phosphatidylinositol 3'-kinase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2530-9, (1999) (PubMed).

Rodewald, Dessing, Dvorak, Galli: "Identification of a committed precursor for the mast cell lineage." in: Science (New York, N.Y.), Vol. 271, Issue 5250, pp. 818-22, (1996) (PubMed).

Yamamoto, Yasumizu, Amou, Watanabe, Nishio, Toki, Fukuhara, Ikehara: "Characterization of peripheral blood stem cells in mice." in: Blood, Vol. 88, Issue 2, pp. 445-54, (1996) (PubMed).

Rodewald, Kretzschmar, Swat, Takeda: "Intrathymically expressed c-kit ligand (stem cell factor) is a major factor driving expansion of very immature thymocytes in vivo." in: Immunity, Vol. 3, Issue 3, pp. 313-9, (1995) (PubMed).

Rodewald, Kretzschmar, Takeda, Hohl, Dessing: "Identification of pro-thymocytes in murine fetal blood: T lineage commitment can precede thymus colonization." in: The EMBO journal, Vol. 13, Issue 18, pp. 4229-40, (1994) (PubMed).

Godfrey, Kennedy, Mombaerts, Tonegawa, Zlotnik: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, (1994) (PubMed).

Moore, Huang, Terstappen, Bennett, Kumar: "Expression of CD43 on murine and human pluripotent hematopoietic stem cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 4978-87, (1994) (PubMed).

Rico-Vargas, Weiskopf, Nishikawa, Osmond: "c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2845-52, (1994) (PubMed).

Valent: "The riddle of the mast cell: kit(CD117)-ligand as the missing link?" in: Immunology today, Vol. 15, Issue 3, pp. 111-4, (1994) (PubMed).

Katayama, Shih, Nishikawa, Kina, Clark, Ogawa: "Stage-specific expression of c-kit protein by murine hematopoietic progenitors." in: Blood, Vol. 82, Issue 8, pp. 2353-60, (1993) (PubMed).

Matsuzaki, Gyotoku, Ogawa, Nishikawa, Katsura, Gachelin, Nakauchi: "Characterization of c-kit positive intrathymic stem cells that are restricted to lymphoid differentiation." in: The Journal of experimental medicine, Vol. 178, Issue 4, pp. 1283-92, (1993) (PubMed).

Orlic, Fischer, Nishikawa, Nienhuis, Bodine: "Purification and characterization of heterogeneous pluripotent hematopoietic stem cell populations expressing high levels of c-kit receptor." in: Blood, Vol. 82, Issue 3, pp. 762-70, (1993) (PubMed).

Godfrey, Zlotnik, Suda: "Phenotypic and functional characterization of c-kit expression during intrathymic T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2281-5, (1992) (PubMed).

Nishikawa, Kusakabe, Yoshinaga, Ogawa, Hayashi, Kunisada, Era, Sakakura, Nishikawa: "In utero manipulation of coat color formation by a monoclonal anti-c-kit antibody: two distinct waves of c-kit-dependency during melanocyte development." in: The EMBO journal, Vol. 10, Issue 8, pp. 2111-8, (1991) (PubMed).

Ogawa, Matsuzaki, Nishikawa, Hayashi, Kunisada, Sudo, Kina, Nakauchi, Nishikawa: "Expression and function of c-kit in hemopoietic progenitor cells." in: The Journal of experimental medicine, Vol. 174, Issue 1, pp. 63-71, (1991) (PubMed).

Zsebo, Williams, Geissler, Broudy, Martin, Atkins, Hsu, Birkett, Okino, Murdock: "Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor." in: Cell, Vol. 63, Issue 1, pp. 213-24, (1990) (PubMed).

Anderson, Lyman, Baird, Wignall, Eisenman, Rauch, March, Boswell, Gimpel, Cosman: "Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms." in: Cell, Vol. 63, Issue 1, pp. 235-43, (1990) (PubMed).

Huang, Nocka, Beier, Chu, Buck, Lahm, Wellner, Leder, Besmer: "The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus." in: Cell, Vol. 63, Issue 1, pp. 225-33, (1990) (PubMed).

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: CD117

Background: The ACK45 antibody reacts with CD117 (c-Kit), a transmembrane tyrosine-kinase receptor which is encoded by the Kit gene (formerly dominant white spotting, W). The c-Kit ligand (also known as steel factor, stem cell factor, and mast-cell growth factor) encoded by the Kitl gene (formerly steel, Sl), is a co-mitogen for hematopoietic stem cells and the myeloerythroid progenitors and a mast-cell differentiation factor. The Kit[W] and Kitl[Sl] mutant alleles have similar pleiotropic effects on the development of melanocytes, germ cells, and the hematopoietic system. In the adult bone marrow, CD117 is expressed on hematopoietic progenitor cells, including the CD90 (Thy-1)[lo] TER-119[-] CD45R/B220[-] CD11b (Mac-1)[-] Ly-6G (Gr-1)[-] CD4[-] CD8[-] Sca-1 (Ly-6A/E)[+] multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and precursors of B and T lymphocytes. This widespread expression of CD117 in hematopoietic precursors is consistent with the participation of c-Kit and its ligand in the regulation of several hematopoietic lineages. Intrathymic expression of c-Kit and c-Kit ligand suggest that CD117 is also involved in the regulation of some events during the development of T lymphocytes. CD117 is also expressed by mast cells. The expression of CD117 has been included in protocols to isolate hematopoietic progenitors from fetal and adult peripheral blood. The specificity and functional activity of mAb ACK45 is very similar to that of the published antibody ACK22, it is reported to block the action of c-Kit in vitro and in vivo. This antibody is routinely tested by flow cytometric analysis.
Synonyms: c-Kit

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity