IL-10 antibody

Catalog No. ABIN1176986

Product Details anti-IL-10 Antibody

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: ELISA, Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-10. Neutralization activity may be measured with a proliferation assay using 5 ng/mL recombinant mouse IL-10 (Cat. No. 550070) and 0.1 ng/mL recombinant mouse IL-4 (Cat. No. 550067) to co-stimulate MC/9 cells at 1x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with the recombinant mouse IL-10 for 60 min).
50% Neutralization (ND50) at 2 - 8 ng/mL
>95% Neutralization 3 - 25 µg/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse IL-10

Clone: JES5-2A5

Isotype: IgG1 kappa

Application Details

Application Notes: ELISA: The purified format of the JES5-2A5 antibody (Cat. No. 551215) has been reported to be useful as a capture antibody in sandwich ELISA for mouse IL-10. Purified JES5-2A5 antibody can be paired with the biotinylated SXC-1 antibody (Cat. No. 554423) or biotinylated JES5-16E3 antibody (Cat. No. 554465) and recombinant mouse IL-10 (Cat. No. 550070) as the standard. Purified JES5-2A5 antibody should be titrated (suggested 2-6 µg/mL) to determine the optimal concentration for ELISA capture. An appropriate range of concentrations of the mouse IL-10 standard for obtaining a linear standard curve is 2 ng/mL to 15 pg/mL mouse IL-10.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-10 antibody (ABIN1176986)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, (1995) (PubMed).

Sarawar, Sangster, Coffman, Doherty: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, (1994) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10 (IL10 Products)

Synonyms: CSIF antibody, GVHDS antibody, IL-10 antibody, IL10A antibody, TGIF antibody, Il-10 antibody, IL10X antibody, interleukin 10 antibody, IL10 antibody, Il10 antibody

Background: The JES5-2A5 antibody reacts with mouse IL-10. The immunogen used to generate the JES5-2A5 hybridoma was E. coli-expressed mouse IL-10. This is a neutralizing antibody.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints