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TEK antibody (pTyr992) (Alexa Fluor 647)

TEK Reactivity: Human, Mouse ICS Host: Mouse Monoclonal K91-860 Alexa Fluor 647
Catalog No. ABIN1177217
  • Target See all TEK Antibodies
    TEK (TEK Tyrosine Kinase, Endothelial (TEK))
    Binding Specificity
    • 19
    • 19
    • 16
    • 11
    • 9
    • 8
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr992
    Reactivity
    • 136
    • 66
    • 49
    • 22
    • 4
    • 3
    Human, Mouse
    Host
    • 128
    • 17
    • 3
    • 2
    • 2
    Mouse
    Clonality
    • 134
    • 18
    Monoclonal
    Conjugate
    • 70
    • 10
    • 9
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    This TEK antibody is conjugated to Alexa Fluor 647
    Application
    • 80
    • 59
    • 40
    • 40
    • 36
    • 27
    • 14
    • 13
    • 12
    • 8
    • 8
    • 6
    • 4
    • 2
    • 1
    • 1
    Intracellular Staining (ICS)
    Brand
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Phosphorylated Human TIE2 Peptide
    Clone
    K91-860
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product TEK Primary Antibody
  • Application Notes
    Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.
    Sample Volume
    20 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Audero, Cascone, Maniero, Napione, Arese, Lanfrancone, Bussolino: "Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cells." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 13224-33, (2004) (PubMed).

    Peters, Kontos, Lin, Wong, Rao, Huang, Dewhirst, Sankar: "Functional significance of Tie2 signaling in the adult vasculature." in: Recent progress in hormone research, Vol. 59, pp. 51-71, (2004) (PubMed).

    DeBusk, Hallahan, Lin: "Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway." in: Experimental cell research, Vol. 298, Issue 1, pp. 167-77, (2004) (PubMed).

    Jones, Iljin, Dumont, Alitalo: "Tie receptors: new modulators of angiogenic and lymphangiogenic responses." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 257-67, (2001) (PubMed).

    Murray, Padrique, Pinko, McTigue: "Mechanistic effects of autophosphorylation on receptor tyrosine kinase catalysis: enzymatic characterization of Tie2 and phospho-Tie2." in: Biochemistry, Vol. 40, Issue 34, pp. 10243-53, (2001) (PubMed).

    Edgell, McDonald, Graham: "Permanent cell line expressing human factor VIII-related antigen established by hybridization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 12, pp. 3734-7, (1983) (PubMed).

  • Target
    TEK (TEK Tyrosine Kinase, Endothelial (TEK))
    Alternative Name
    TIE2 (TEK Products)
    Synonyms
    TEK antibody, DKFZp469E064 antibody, AA517024 antibody, Cd202b antibody, Hyk antibody, Tie2 antibody, tie-2 antibody, CD202B antibody, TIE-2 antibody, TIE2 antibody, VMCM antibody, VMCM1 antibody, Tie-2 antibody, tie2 antibody, TEK receptor tyrosine kinase antibody, angiopoietin-1 receptor antibody, TEK tyrosine kinase, endothelial antibody, TEK antibody, LOC100408334 antibody, tek antibody, Tek antibody
    Background
    TIE2 (Tyrosine kinase with Immunoglobulin-like and EGF-like domains 2), also known as TEK (Tunical Endothelial Kinase), is an endothelial and hematopoietic cell-specific receptor tyrosine kinase (RTK) that is critical to the development and maintenance of the vasculature and highly conserved among vertebrate species. The angiopoietins are ligands of TIE2, and the abnormal vascular growth that often occurs in solid tumors is a result of disruptions in the coordinated actions of the angiopoietins, TIE2, and the closely related TIE1 RTK. Upon activation by angiopoietins, TIE2 autophosphorylates at least 2 tyrosines in its protein kinase domain and at least 3 tyrosines in its C-terminal tail. The K91-860 monoclonal antibody recognizes the phosphorylated tyrosine 992 (Y992) in the protein kinase domain of activated TIE2. The orthologous phosphorylation site in mouse TIE2 is Y990.
    Synonyms: TEK, VMCM, CD202b
    Pathways
    RTK Signaling, Growth Factor Binding
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