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STAT5A antibody (pTyr694) (PE-CF594)

STAT5A Reactivity: Human ICS Host: Mouse Monoclonal 47-Stat5 PE-CF594
Catalog No. ABIN1177255
  • Target See all STAT5A Antibodies
    STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
    Binding Specificity
    • 47
    • 24
    • 17
    • 15
    • 15
    • 14
    • 9
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr694
    Reactivity
    • 191
    • 101
    • 75
    • 25
    • 9
    • 8
    • 7
    • 6
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 176
    • 18
    • 2
    Mouse
    Clonality
    • 156
    • 40
    Monoclonal
    Conjugate
    • 114
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    This STAT5A antibody is conjugated to PE-CF594
    Application
    • 159
    • 69
    • 57
    • 40
    • 39
    • 30
    • 23
    • 21
    • 19
    • 13
    • 10
    • 8
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Intracellular Staining (ICS)
    Brand
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Phosphorylated Sheep Stat5 Peptide
    Clone
    47-Stat5
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product STAT5A Primary Antibody
  • Application Notes
    This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I).
    Comment

    Signal transducer, activator of transcription 5, MGF, MPF

    Sample Volume
    5 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.
  • Van De Wiele, Marino, Murray, Vo, Whetsell, Teague: "Thymocytes between the beta-selection and positive selection checkpoints are nonresponsive to IL-7 as assessed by STAT-5 phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4235-44, (2004) (PubMed).

    Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).

    Liu, Gaffen, Goldsmith: "JAK/STAT signaling by cytokine receptors." in: Current opinion in immunology, Vol. 10, Issue 3, pp. 271-8, (1998) (PubMed).

    Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, (1994) (PubMed).

  • Target
    STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
    Alternative Name
    Stat5 (STAT5A Products)
    Synonyms
    MGF antibody, STAT5 antibody, AA959963 antibody, Stat5 antibody, STAT5A/MGF antibody, STATA5 antibody, STAT5A antibody, stat5 antibody, stat5b antibody, signal transducer and activator of transcription 5A antibody, signal transducer and activator of transcription 5a antibody, STAT5A antibody, Stat5a antibody, stat5a antibody, LOC100348518 antibody, LOC100716811 antibody
    Background
    Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699. This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
    Synonyms: Signal transducer and activator of transcription 5, MGF, MPF
    Pathways
    JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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