PLK1 antibody (pThr210)

Catalog No. ABIN117982

This Rabbit Polyclonal antibody specifically detects PLK1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Xenopus laevis, Dog, Zebrafish (Danio rerio), Pig, Chimpanzee and Chicken.

Quick Overview for PLK1 antibody (pThr210) (ABIN117982)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Mouse, Rat, Xenopus laevis, Dog, Zebrafish (Danio rerio), Pig, Chimpanzee, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PLK1 Antibody

Binding Specificity: AA 205-214, pThr210

Sequence: 1 msaavtagkl arapadpgka gvpgvaapga paaappakei pevlvdprsr rryvrgrflg 61 kggfakcfei sdadtkevfa gkivpkslll kphqrekmsm eisihrslah qhvvgfhgff 121 edndfvfvvl elcrrrslle lhkrrkalte pearyylrqi vlgcqylhrn rvihrdlklg 181 nlflnedlev kigdfglatk veydgerkkt lcgtpnyiap evlskkghsf evdvwsigci 241 mytllvgkpp fetsclkety lrikkneysi pkhinpvaas liqkmlqtdp tarptinell 301 ndefftsgyi parlpitclt ipprfsiaps sldpsnrkpl tvlnkglenp lperprekee 361 pvvretgevv dchlsdmlqq lhsvnaskps erglvrqeea edpacipifw vskwvdysdk 421 yglgyqlcdn svgvlfndst rlilyndgds lqyierdgte syltvsshpn slmkkitllk 481 yfrnymsehl lkaganitpr egdelarlpy lrtwfrtrsa iilhlsngsv qinffqdhtk 541 lilcplmaav tyidekrdfr tyrlslleey gcckelasrl ryartmvdkl lssrsasnrl 601 kas

Specificity: This antibody is specific for phosphorylated Human Plk-1 protein at the pThr210 residue. Cross reactivity with Plk-1 protein homologues from C.elgans and Honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Pig, Chicken, Mouse, Rat, Xenopus, Dog, Mosquito, Zebrafish, Starfish, Sea urchin and Fruit Fly.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities

Immunogen: Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein

Application Details

Application Notes: ELISA (1/3,000-1/12,000). Immunohistochemistry on Paraffin Sections (1/200-1/1,000). This antibody is positive byIHC on kidney, liver, cancer, thyroid and lymphocyte tissue. Western blot (1/200-1/2,000). Expect a band approximately 68 kDa in size correspondingto Plk-1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.Synonyms: PLK-1, Polo-like kinase 1, STPK13, Serine/threonine-protein kinase PLK1

Gene ID: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Cell Division Cycle, M Phase