CD11b antibody

Catalog No. ABIN118145

The Rat Monoclonal anti-CD11b antibody is suitable to detect CD11b in samples from Human, Mouse and Rabbit. It has been validated for FACS, IP, IHC (fro) and IHC (p).

Quick Overview for CD11b antibody (ABIN118145)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Rabbit

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: M1-70

Product Details anti-CD11b Antibody

Purification: Ion Exchange Chromatography

Immunogen: T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Immunoprecipitation. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections. Flow cytometry: 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Clone M1/70.15 has been reported to block iC3b binding to its receptor. ABIN118139 is recommended for this purpose.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: CD11b is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 10090

UniProt: P05555

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation