ITGA4 antibody (FITC)

Catalog No. ABIN118225

This Rat Monoclonal antibody specifically detects ITGA4 in FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for ITGA4 antibody (FITC) (ABIN118225)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: PS-2

Product Details anti-ITGA4 Antibody (FITC)

Specificity: This antibody recognises murine alpha 4 integrin (CD49d). Clone PS/2 has also been reported to block the binding of CD49d to its ligands. We recommend the use of SM037LE for this purpose.

Purification: Affinity chromatography on Protein G.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/5-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d / ITGA4

Background: Integrin α4 (also called CD49d) is a ~150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin α4 forms heterodimers with integrins β1 and β7. Integrin α4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin α4 promotes cell migration and inhibits cell spreading and contractility. Integrin α4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin α4 function has become an important target for drug discovery. Phosphorylation of the serine 988 residue on the integrin α4 cytoplasmic tail inhibits its interaction with the focal adhesion complex-associated protein paxillin, and modulates its effects on cell motility and spreading.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Gene ID: 3676

NCBI Accession: NP_000876

UniProt: P13612

Pathways: Integrin Complex