Integrin beta 2 antibody (FITC)

Catalog No. ABIN118552

This Rat Monoclonal antibody specifically detects Integrin beta 2 in FACS. It exhibits reactivity toward Human and Dog.

Quick Overview for Integrin beta 2 antibody (FITC) (ABIN118552)

Target: Integrin beta 2 (ITGB2)

Reactivity: Human, Dog

Host: Rat

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: YFC118-3

Product Details anti-Integrin beta 2 Antibody (FITC)

Specificity: This antibody was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen as recognising the CD18 antigen. The main cellular reactivity of antibody YFC 118.3 is a strong reactivity with Leucocytes (platelets negative).

Purification: Affinity chromatography on Protein G

Immunogen: Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the Y3/Ag.1.2.3 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18 / ITGB2

Background: The CD18 antigen is an integral membrane glycoprotein of 95kD molecular weight - known as the beta chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules. The CD18 Molecule is the receptor for ICAM-1 and is thus important for cell adhesion.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gene ID: 3689

NCBI Accession: NP_000202

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation