YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen as recognising the CD18 antigen. The main cellular reactivity of antibody YFC 118.3 is a strong reactivity with Leucocytes (platelets negative). YFC118.3 blocks homotypic adhesion and other lymphocyte functions which involve the LFA-1 antigen.
Purification
Affinity chromatography on Protein G
Immunogen
Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the Y3/Ag.1.2.3 rat myeloma cell line.
Flow cytometry: Use 10 μL of neat antibody to label 100 μL whole blood or 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
SM1076R: Restore with 1 mL distilled water. SM1076RT: Restore with 25 μL distilled water.
Buffer
PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The CD18 antigen is an integral membrane glycoprotein of 95kD molecular weight - known as the b chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules. The CD18 Molecule is the receptor for ICAM-1 and is thus important for cell adhesion.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7