His Tag antibody (Biotin)

Catalog No. ABIN119351

The Mouse Monoclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB, IP, IHC (fro) and EIA.

Quick Overview for His Tag antibody (Biotin) (ABIN119351)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: AD1-1-10

Product Details anti-His Tag Antibody (Biotin)

Sequence: HHHHHH

Purification: Protein G affinity chromatography

Immunogen: PAX6 transcription factor linked to histidine tag

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/1000. Western Blot: 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is routinely tested in ELISA using histidine tagged ubiquitin and in Western blotting using histidine tagged recombinant protein control.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for His Tag

Target: His Tag

Alternative Name: 6xHistidine Epitope Tag

Target Type: Tag