GZMB antibody

Catalog No. ABIN119565

The Mouse Monoclonal anti-GZMB antibody (Clone GB11) (ABIN119565) specifically detects GZMB in FACS, IF, IP, IHC (fro) and EIA. The antibody is reactive with Human, Monkey and Chimpanzee samples.

Quick Overview for GZMB antibody (ABIN119565)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human, Monkey, Chimpanzee

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: GB11

Product Details anti-GZMB Antibody

Specificity: This antibody recognises the serine protease Granzyme B. This antibody has been reported to function as a Capture reagent in ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 (SM1805B) as detection reagent.

Purification: Affinity chromatography on Protein A

Immunogen: Purified human Granzyme B. Remarks: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections. Flow cytometry: 1/10 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood, Membrane permeabilisation is required for this application. Immunoprecipitation. ELISA: 2 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Tris buffered saline containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Background: Ganzyme B is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death.Synonyms: CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Granzyme-2, Lymphocyte protease, SECT, T-cell serine protease 1-3E

Gene ID: 3002

NCBI Accession: NP_004122

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis