Calcitonin Receptor antibody (Cytoplasmic Domain)

Catalog No. ABIN119693

The Mouse Monoclonal anti-Calcitonin Receptor antibody is suitable to detect Calcitonin Receptor in samples from Human and Rabbit. It has been validated for WB, IF, FACS, IHC (p), IHC (fro) and EIA.

Quick Overview for Calcitonin Receptor antibody (Cytoplasmic Domain) (ABIN119693)

Target: Calcitonin Receptor (CALCR)

Reactivity: Human, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calcitonin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 31-01-14

Product Details anti-Calcitonin Receptor Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: This antibody recognizes the Calcitonin Receptor. Clone 31/01 recognizes an epitope within the cytoplasmic domain that is common to both C1a and C1b Human isoforms.

Purification: Affinity Chromatography on Protein A.

Immunogen: Synthetic peptide derived from a sequence within the cytoplasmic domain of Human Calcitonin Receptor. Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1/1000 - 1/5000. Immunohistochemistry on paraffin sections: 1/50 - 1/500, we recommend the use of 4 % paraformaldehyde fixation, rather than formalin fixation, This product does not require protein digestion pre-treatment of paraffin embedded sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Recommended Positive Control: Kidney.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Calcitonin Receptor

Target: Calcitonin Receptor (CALCR)

Alternative Name: Calcitonin Receptor

Background: Calcitonin receptor is a membrane protein with seven transmembrane domains that is coupled to G protein messenger systems. The calcitonin receptor is expressed in kidney, central and peripheral nervous systems and on osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.Synonyms: CALCR, CT-R, CTR

Gene ID: 799

NCBI Accession: NP_001158209

UniProt: P30988

Pathways: cAMP Metabolic Process